The plan is to develop the nanoparticles separation technology for biotoxicity testing in environmental water. A total of eighteen water samples were taken: municipal wastewater, paper industry, mechanical processing park, sheet metal painting industry, semiconductor science park, ceramics, cosmetics raw materials manufacturing, electronic industry, leather processing and manufacturing, optical electronics industry and natural water. The separation of the asymmetric flow field field (AF4) system was carried out after the dynamic light scattering (DLS) instrument screen the nanoparticles in the water. The correlation between the DLS and the AF4 separation system was evaluated. AF4 separation system was used to study the size distribution of nanoparticles in water samples. The composition of organic components / inorganic metals was also studied by pyrolysis gas chromatography mass spectrometry (GC-MS) / inductively coupled plasma mass spectrometer (ICP-MS), and providing the draft method for separation of nanoparticles in water. The DLS detection of 10 in 18 water samples is higher than the screening standard in 0.1µm filter. The detected value of 12 samples is higher than the screening standard for 0.22µm filter. Most of the samples, the asymmetric flow field field (AF4) system separates successfully from small to large particles in water mobile phase and good reproducibility. Different vials might be signal differences but does not affect the distribution of particle size. Theρ(Rg / Rh) value greater than 1 shows that the nanoparticles of these water samples are loose structure except for Paper industry effluent. Theρvalue of Paper industry effluent 0.6 to 0.8 indicates the structure close to the solid sphere. Aarsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), lead (Pb), silver (Ag), zinc (Zn), iron (Fe) are analyzed by inductively coupled plasma mass spectrometry (ICP-MS) and are also manganese (Mn) / nickel (Ni) / selenium (Se) / calcium (Ca) / magnesium (Mg), a total of thirteen kinds of metal / elemental components. As, Cu, Zn, Fe, Mn, Ni, Ca and Mg are tested among these water samples. Total Organic Carbon (TOC), Volatile Organic Compound (VOC) in water and organic composition analysis by pyrolysis gas chromatography mass spectrometry were also done. A total of six water samples of municipal wastewater, sheet metal painting industry and mechanical processing park are tested for biological toxicity. The original water of sheet metal painting industry affects the cell viability. We could estimate the cause of cells survival rate decline with the particle size and organic / inorganic components analysis and achieve the development of nano-material separation technology for biological toxicity testing in environmental water.

Asymmetric Flow Field-Flow-Fractionation (AF4)、Multi-Angle static Light Scattering (MALS)、Environmental Nanoparticle